Katharina Hofmann

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B6 Activation of human antigen presenting cells by mycobacterial glycolipids

Principal investigator
Roland Lang

Mentor
Anja Lührmann

Activation of human antigen presenting cells by mycobacterial glycolipids

The use of recombinant proteins as subunit vaccines is a safe and attractive strategy for immunization. But, in order to achieve an effective immune response, such subunit vaccines need the application of adjuvants that activate antigen-presenting cells of the innate immune system.
The mycobacterial cord factor of the cell wall, Trehalose-6.6-dimycolate (TDM), and its synthetic analogue Trehalose-6.6-dibehenate (TDB) are effective adjuvants for generating Th1 and Th17 cellular immunity. This type of immune responses is associated with protection against intracellular pathogens such as Mycobacterium tuberculosis. The activation of macrophages and dendritic cells requires the Syk-Card9-Bcl10-Malt1-dependent signalling pathway. The identification of the receptors involved in the recognition of the glycolipid adjuvants TDB and cord factor is the subject of current investigation.
To date, the inflammatory and adjuvant properties of TDM and TDB have been mostly investigated in the mouse system. The goal of this project is to explore the effects of TDB and cord factor on human antigen-presenting cells and to determine whether the same signaling components are involved as in the murine system.

Figure: PBMCs with GM-CSF-matured macrophages; stimulated with TDB

 

Publications

 

Presentations

September 2010 2nd Annual Retreat, Erlangen School of Molecular Communication, Kloster Banz, Bad Staffelstein, Germany
Activation of antigen presenting cells by mycobacterial glycolipids”
Talk