Lisa Ott

Suche


B5 Analysis of virulence factors of Corynebacterium diphtheriae

Principal investigator
Andreas Burkovski

Mentor
Andreas Goldwich

PhD exam: 19.12.2012

Analysis of virulence factors of Corynebacterium diphtheriae

In frame of the doctoral project, adhesion of different Corynebacterium diphtheriae wild type strains to the human pharyngeal cell line Detroit562 and invasion of these strains into Detroit562 cells are investigated. As a global approach to identify the molecular basis of strain-specific differences observed in these processes, surface proteomes of several non-toxigenic C. diphtheriae strains are analyzed by 2D-PAGE and MALDI-ToF-MS to identify differentially expressed proteins, which might function as putative new virulence factors.
In addition, mutagenesis studies are carried out based on data of already identified surface proteins of C. diphtheriae. As a first result, it could be shown that the putative invasion-associated protein DIP1281 is involved in the organization of the
C. diphtheriae outer cell surface and in accurate cell division, and that mutagenesis of the corresponding gene results in a complete loss of adhesion.
While the described approaches of the PhD project aim to characterize pathogen proteins and their function in the attack of human cells, in parallel a second part of the project focuses on the characterization of the host response to this challenge. For this purpose, reporter cell lines for different host signal pathways are established in cooperation with Prof. A. Ensser. These cells are infected with C. diphtheriae and the response of different signal pathways is analyzed by a luciferase assay.

Figure: LIVE/DEAD staining of C. diphtheriae mutant ISS3319::pk18mob`DIP1281; dead bacteria in red (propidium jodide), alive bacteria in green (SYTO9). Bacteria build long chains, due to loss of cell division.

 

Publications

Ott, L., Scholz, B., Höller, M., Hasselt, K., Ensser, A. and Burkovski, A. (2012). Induction of the NFκ-B signal transduction pathway in response to Corynebacterium diphtheriae infection. Microbiology (in press).

Rheinlaender, J., Gräbner, A., Ott, L., Burkovski, A. and Schäffer, T. E. (2012). Contour and persistence length of Corynebacterium diphtheriae pili by atomic force microscopy. Eur Biophys J 41(6), 561-570.

Ott, L., McKenzie, A., Baltazar, M. T., Britting, S., Bischof, A., Burkovski, A. and Hoskisson, P. (2012). Evaluation of invertebrate infection models for pathogenic corynebacteria FEMS Immunobiol Med Microbiol, 65(3), 413-421.

Ochrombel, I., Ott, L., Krämer, R., Burkovski, A. and Marin, K. (2011). Impact of improved potassium accumulation on pH homeostasis, membrane potential adjustment and survival of Corynebacterium glutamicum. Biochim Biophys Acta 1807, 444-450.

Trost, E., Ott, L., Schneider, J., Schröder, J., Jaenicke, S., Goesmann, A., Hüsemann, P., Stoye, J., Alves Dorella, F., Rocha, F. S., de Castro Soares, S., D'Afonseca, V., Miyoshi, A., Ruiz, J., Silva, A., Azevedo, V., Burkovski, A., Guiso, N., Join-Lambert, O. F., Kayal, S. and Tauch, A. (2010). The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence. BMC Genomics 11, 728.

Ott, L., Höller, M., Rheinlaender, J., Schäffer, T. E., Hensel, M. and Burkovski, A. (2010). Strain-specific differences in pili formation and the interaction of Corynebacterium diphtheriae with host cells. BMC Microbiol 10:257.

Ott, L., Höller, M., Gerlach , R.G., Hensel, M., Rheinländer, J., Schäffer, T. E. and Burkovski, A. (2010). Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells. BMC Microbiol 10:2.

Amon, J., Bräu, T., Grimrath, A., Hänssler, E., Hasselt K., Höller, M., Jessberger, N., Ott, L., Szököl, J., Titgemeyer, F. and Burkovski, A. (2008). Nitrogen control in Mycobacterium smegmatis: nitrogen-dependent expression of ammonium transport and assimilation proteins depends on the OmpR-type regulator GlnR. J Bacteriol 190, 7108-7116.

       

Presentations

July 2012 4th Annual Retreat, Erlangen School of Molecular Communication, Kloster Banz, Bad Staffelstein, Germany
Evaluation of invertebrate infection models for pathogenic corynebacteria
Talk
     
March 2012 Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie, Tübingen, Germany
Evaluation of invertebrate infection models for pathogenic corynebacteria
Poster
     
October 2011 First International SFB 796 Conference: Mechanisms of viral host cell manipulations: from plants to humans, Bamberg, Germany
Strain-specific differences in the interaction of Corynebacterium diphtheriae with host cells
Poster
     
July 2011 3rd Annual Retreat, Erlangen School of Molecular Communication, Kloster Banz, Bad Staffelstein, Germany
Interaction of Corynebacterium diphtheriae with host cells
Talk and Poster
     
May 2011 Mol Micro Meeting Würzburg, Würzburg, Germany
Strain-specific differences in pili-formation and interaction of Corynebacterium diphtheriae with host cells
Poster
     
April 2011 Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie, Karlsruhe, Germany
Strain-specific differences in pili-formation and interaction of Corynebacterium diphtheriae with host cells
Poster
     
September 2010 2nd Annual Retreat, Erlangen School of Molecular Communication, Kloster Banz, Bad Staffelstein, Germany
Adhesion and Invasion of Corynebacterium diphtheriae
Talk
     
March 2010 3th joint Conference DGHM and VAAM, Hannover, Germany
Strain-specific differences in the interaction of Corynebacterium diphtheriae with host cells
Poster
     
September 2009 First Annual Retreat, Erlangen School of Molecular Communication, Schloss Atzelsberg, Atzelsberg, Germany
Adhesion and Invasion of Corynebacterium diphtheriae
Talk