Walter Schäfer

Suche


B8 Functional analysis of Coxiella burnetii effector proteins

Principal investigator
Anja Lührmann

Mentor
Steffen Backert

Functional Analysis of Coxiella burnetii type IV effector-induced inhibition of host cell apoptosis

Coxiella burnetii is the Gram-negative obligate intracellular pathogen that causes Q fever, a zoonotic disease. The transmission of Q fever occurs through inhalation of aerosolized soil or animal products contaminated with C. burnetii. The most common reservoirs for this pathogen are cattle, sheep and goats but it also infects many birds and pets as well as arthropods. Q fever is clinically manifested as a flu-like illness, but can also develop into severe pneumonia, hepatitis or endocarditis. It was shown that C. burnetii expresses a type IV secretion system (T4SS), which is essential for the intracellular replication and survival. Therefore, it was concluded that the activity of effector proteins, translocated by the T4SS into the host cells, is crucial for bacterial survival and replication. However, the function of the so far 110 identified C. burnetii effector proteins is unknown, which the exception of AnkG and CaeB. We have shown that the C. burnetii T4SS effector proteins AnkG and CaeB inhibit intrinsic apoptosis However, the precise mode of action of these effector proteins is still unknown. Thus, I aim to study the molecular activity of AnkG and CaeB.

 

Figure:This scheme describes the mechanisms by which bacterial pathogens inhibit apoptosis at different points along the apoptotic pathway. Red lines indicate inhibition in the pathway, while green arrows indicate activation. The bacterial proteins that specifically participate in apoptosis inhibition are shown, where known. Adapted from (Faherty & Maurelli, 2008).

 

Publications

Schäfer, W., Eckart, R. A., Schmid, B., Cagköylü, H., Hof, K., Muller, Y. A., Amin, B., and Lührmann, A. (2016) Nuclear trafficking of the anti-apoptotic Coxiella burnetii effector protein AnkG requires binding to p32 and Importin-α1. Cellular Microbiology, doi: 10.1111/cmi.12634.

 

Presentations 

October 2016

8th Annual Retreat, Erlangen School of Molecular Communication, Schloss Schney, Lichtenfels, Germany
”Nuclear trafficking of the anti-apoptotic Coxiella burnetii effector protein AnkG requires binding to p32 and Importin-α1”

Talk
     
June 2016

28th Meeting of the American Society for Rickettsiology Big Sky Montana, USA
”Nuclear trafficking of the anti-apoptotic Coxiella burnetii effector protein AnkG requires binding to p32 and Importin-α1”

Talk
     
October 2015

2nd International SFB 796 Conference: Mechanisms of microbial host cell manipulation: From plants to humans, Erlangen, Germany
”Comparison of the activity of the effector AnkG from different Coxiella burnetii strains”

Poster
     
September 2015

67. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie DGHM, Münster, Germany
”Comparison of the activity of the effector AnkG from different Coxiella burnetii strains”

Poster
     
July 2015 7th Annual Retreat, Erlangen School of Molecular Communication, Schloss Hirschberg, Beilngries, Germany
”Identification of the anti-apoptotic region of the Coxiella burnetii effector AnkG”
Poster
     
October 2014 66. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie, DGHM, Dresden, Germany
“Identification of the anti-apoptotic region of the Coxiella burnetii effector AnkG”
Poster
     
July 2014 6th Annual Retreat, Erlangen School of Molecular Communication, Kloster Banz, Bad Staffelstein, Germany
“Functional Analysis of AnkG-induced inhibition of host cell apoptosis”
Talk