Haina Zhang

Suche


C2 Functional analysis of the interaction between host chaperones and viral transport proteins

Principal investigator
Uwe Sonnewald

Mentor
Benedikt Kost

Influence of abiotic stress on virus induced signal transduction networks

Due to the current climate changes, plants are not only attacked by various pathogens, but also exposed to a wide range of environmental stress, such as heat, cold, drought, salinity, solar radiation, and nutrient deficiency. To date, most studies on plant responses to environmental changes only focus on single or double abiotic stress factors, which fail to explain complex defensing networks to combined stress situations. Previous studies have shown that each stress treatment activates a distinct signaling network in Arabidopsis (Arabidopsis thaliana). Moreover, data also showed that different TIR-NBS-LRR genes and transcription factors are activated under single and multifactorial stress situations. Thus, in the future work, we will focus on the influence of combined abiotic stress, like heat and drought on virus (TuMV) induced signal transduction networks, using in silico analysis to select the potential candidates in the signal transduction networks under triple stress, applying transcriptome and metabolome analysis to compare the molecular and biochemical responses between T-DNA knock-out mutants and ecotypes, and using GFP fusion proteins to study the localization of candidate proteins within the cells. Both knock-out lines and overexpressing transgenic plants will be challenged with multiple stresses, to elucidate the role of the selected candidate genes in plant defense

Figure:Network of genes under virus and triple stresses. (A) Gene network of receptor proteins (yellow), transcription factors (blue), and signaling proteins (green) under virus stress. (B) Gene network of receptor proteins (yellow), signaling proteins (blue) and transcription factors (green) under heat, drought, and virus stresses.

 

 

 

Publications

 

 

Presentations

October 2016 8th Annual Retreat, Erlangen School of Molecular Communication, Schloss Schney, Lichtenfels, Germany
”The Arabidopsis protein Rap2.9 - a central integrator mediating plant growth and defense response to abiotic and biotic stress”
Poster
     
October 2015 2nd International SFB 796 Conference: Mechanisms of microbial host cell manipulation: From plants to humans, Erlangen, Germany
”Identification of crucial genes mediating biotic and abiotic stress signal transduction networks”
Poster
     
July 2015 7th Annual Retreat, Erlangen School of Molecular Communication, Schloss Hirschberg, Beilngries, Germany
”Influence of abiotic stress on virus induced signal transduction networks”
Poster
     
July 2014 6th Annual Retreat, Erlangen School of Molecular Communication, Kloster Banz, Bad Staffelstein, Germany
"Influence of abiotic stress on virus induced signal transduction networks"
Poster